Well, there are real life loads and there are design loads. We design professionals have to work with design loads. Generally in a house, the first floor contains the living spaces, and the building code requires us to design for 40 pounds per square foot live load. The second floor typically contains bedrooms, and the code permits a design live load of only 30 pounds per square foot for floors containing sleeping rooms. Attic floor loads and roof loads are generally carried directly on walls or beams, and not transmitted to a floor below.
However, if your living spaces are lightly furnished, and your bedrooms have, say, Aunt Mabel's heavy old antiques and lots of them, it is possible that in real life, the loads on the second floor could exceed the loads on the first floor. However, they are not likely to exceed the design live load of 30 pounds per square foot.
For same size beams spanning the same distance, deflection is basically proportional to load, so you could assume that generally, the load on your second floor is actually less than half the 30 pound design live load, when averaged over the entire area carried by the beams.
Architect (NY) and Home Designer (PA)